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master's thesis
Direktno potenciometrijsko određivanje ptijalina u slini
2012. urn:nbn:hr:181:036291
Regušić, Lidija
Josip Juraj Strossmayer University of Osijek
Department of biology

Cite this document

Regušić, L. (2012). Direktno potenciometrijsko određivanje ptijalina u slini (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:036291

Regušić, Lidija. "Direktno potenciometrijsko određivanje ptijalina u slini." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2012. https://urn.nsk.hr/urn:nbn:hr:181:036291

Regušić, Lidija. "Direktno potenciometrijsko određivanje ptijalina u slini." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2012. https://urn.nsk.hr/urn:nbn:hr:181:036291

Regušić, L. (2012). 'Direktno potenciometrijsko određivanje ptijalina u slini', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 13 November 2024, https://urn.nsk.hr/urn:nbn:hr:181:036291

Regušić L. Direktno potenciometrijsko određivanje ptijalina u slini [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2012 [cited 2024 November 13] Available at: https://urn.nsk.hr/urn:nbn:hr:181:036291

L. Regušić, "Direktno potenciometrijsko određivanje ptijalina u slini", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:181:036291

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Metadata
TitleDirektno potenciometrijsko određivanje ptijalina u slini
Title (english)Direct potentiometric determination of salivary amylase
AuthorLidija Regušić
MentorMilan Sak-Bosnar (mentor)
Committee memberElizabeta Has Schon (predsjednik povjerenstva)
Committee memberBerislav Marković (član povjerenstva)
Committee memberMilan Sak-Bosnar (član povjerenstva)
Committee memberRužica Matešić-Puač (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2012-06-20, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Ptijalin je enzim koji se nalazi u sastavu sline gdje katalizira hidrolizu škroba. Nehidrolizirani škrob, čija je koncentracija obrnuto proporcionalna koncentraciji ptijalina, stvara kompleks s trijodidom smanjujući pritom početnu koncentraciju trijodida. Promjena koncentracije trijodidnog iona izaziva promjenu vrijednosti omjera koncentracija redoks para trijodid/jodid, što prema Nernstovom izrazu uzrokuje promjenu redoks potencijala redoks senzora. Ta je promjena u direktnoj korelaciji s koncentracijom ptijalina. U radu je uporabom platinskog redoks senzora provedena direktna potenciometrijska metoda za određivanje aktivnosti ptijalina u biološkim uzorcima sline. U sklopu rada ispitan je utjecaj stresora na aktivnost ptijalina u slini.
Abstract (english)
Salivary alpha amylase (sAA9, an enzyme present in human saliva, catalyses the breakdown of starch. The nonhydrolysed starch, which amount is inversely proportional to the sAA concentration, forms a complex with triiodide reducing accordingly initial triiodide concentration. The change of triiodide ion concentration causes the change of triiodide/iodide redox-couple ratio, which results with the changes of the changes of the electrode potential of a redox-sensor. The changes are directly correlated with sAA concentration. In this paper direct potentiometric method for determination of sAA activity in human saliva was performed. Additionally, sAA response to the different stressors have been investigated.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:036291
Study programmeTitle: Master of Science Graduate University Study Programme in Biology and Chemistry; specializations in: Master Education In Chemistry and Biology
Course: Master Education In Chemistry and Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsClosed access
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Created on2017-01-17 10:40:33