Soil mesofauna is crucial for ecosystems, connecting microfauna and macrofauna in decomposer communities, facilitating organic matter decomposition and nutrient cycling. Mites (Acari) and springtails (Collembola) are common in most soils, with their abundance affected by soil management and amendments. Conventional tillage with heavy machinery often leads to soil compaction, reducing biological activity. In contrast, conservation tillage with minimal disturbance and crop rotation helps maintain biological processes and controls pests. This study aimed to assess the impact of conventional and conservation tillage on mesofauna, focusing on tillage methods, liming, fertilizer application, and GeO2 addition. Conducted on 10x8 m plots with different tillage, liming, and fertilization treatments, the research includes various tillage depths, liming, and four fertilization approaches. Sampling in April and October 2023; organisms were extracted using the Tullgren/Berlese method, scanned in high resolution following the Soil BON method, counted and statistically analyzed. Mites and springtails made up 89% of individuals. Results indicated that conservation tillage, especially deep tillage, enhances mesofauna abundance. Liming positively affects mesofauna, particularly Mesostigmata mites, while the highest organism numbers were observed with reduced amount of fertilizer compared to the recommended quantity, without the addition of GeO2, regardless of soil treatment.