Long – eared owl (Asio otus L. 1758) is a bird from the family of tipical owls (Strigidae) and from the order of owl – like birds (Strigiformes). It is a social bird who spends winter in populated areas gathered around at daytime sleeping area in groups that can contain up to several hundred individuals. The aim of this thesis is to note the locations of daytime sleeping areas of long – eared owl in Osijek and vicinity, as well as analize the climate characteristics of winter 2018/2019 and determine the composition of long – eared owl nutrition. The individuals were counted at three locations in Osijek: Passage of Julije Benešić, area near kindergarden „Sjenčica“ in Sjenjak and Chapel of St. Roch. For their daytime sleeping areas long – eared owls choosed the silver birch (Betula pendula Roth) and cypress (Cupressus sp.). A total of 125 individuals were counted. Winter temperatures 2018/2019 were in the normal range, while due to the lack of precipitation the period was dry. Pellets are undigested residues that contain bones, skulls and teeth of digested animals mixed with hair. In the analyzed pellets the presence of four species of prey were determined: common vole (Microtus arvalis Pallas), striped field mouse (Apodemus agrarius Pallas), house mouse (Mus musculus L.) and undetermined species of mice from the genus Apodemus. The most common species is Microtus arvalis (30 individuals), followed by Apodemus agrarius (nine individuals) and Mus musculus (five individuals) and at last undetermined species of mice from the genus Apodemus (two individuals).