Industrial hemp (Cannabis sativa L.) is in the center of attention due to its comprehensive daily use and numerous bioactive molecules, especially antioxidants that have been proven to improve human health. Light is one of the most important abiotic factors affecting plant growth and development. The aim of this work was to determine how different types of lighting (a combination of LED and QMH light, white and purple LED and fluorescent light) affect the total antioxidant activity, the content of total soluble polyphenols, the concentration of photosynthetic pigments, proteins, the content of total soluble sugars and the amount of dry matter in seedlings of two industrial hemp varieties (Finola and USO 31). The results showed that for growing seedlings, the most optimal type of lighting is a combination of LED and QMH lamps, since the seedlings grown under these conditions showed the highest values of most of the measured antioxidant response parameters. LED light was proven to be the least suitable type of light for growing industrial hemp seedlings. The variety USO 31 showed better antioxidant properties compared to the variety Finola under the given growing conditions