| Abstract | Plastika zbog svojih fizikalno-kemijskih svojstava predstavlja jedan od najsvestranijih materijala današnjice. Antropogenim djelovanjem plastika dolazi u okoliš gdje se pod utjecajem ekoloških čimbenika razgrađuje na čestice različitih veličina. U zadnje vrijeme raste interes za istraživanjem izvora, tipova i koncentracija čestica plastike prisutnih u okolišu, te njihovih djelovanja na ondje prisutne organizme. U ovom radu 2 vrste skokuna: (1) Folsomia candida i (2) Sinella curviseta, bile su izložene trima tipovima mikroplastike: (1) polistirenu-HBCD, (2) škrobnoj plastici i (3) strugotinama automobilskih guma u koncentracijama od 1, 10, 100, 1 000 i 10 000 mg/kg. Skokuni su bili izloženi mikroplastici 28 dana u standardiziranom tlu, poslije čega je učinak mikroplastike na organizme bio ispitan mjerenjem biomarkera na substaničnoj razini biološke organizacije – mjerile su se aktivnosti acetilkolinesteraze (AChE), karboksilesteraze (CES), glutation-S-transferaze (GST) i katalaze (CAT); te koncentracije glutationa (GSH), reaktivnih kisikovih vrsta (ROS), ugljikohidrata i lipida. U obje vrste uočena je značajna promjena u pojedinim mjerenim biomarkerima što može doprinijeti boljem razumijevanju utjecaja mikroplastike na organizme tla. |
| Abstract (english) | Plastic is, due to its physical and chemical properties, one of the most versatile materials today. Due to anthropogenic activity, plastic enters the environment where, under the influence of environmental factors getsbroken down into different sized particles. Recently, there has been a growing interest in researching the sources, types and concentrations of plastic particles present in the environment, and their effects on present organisms. In this study, 2 species of Collembolans: (1) Folsomia candida and (2) Sinella curviseta, were exposed to three types of microplastics: (1) polystyrene-HBCD, (2) starch plastic, and (3) tirewear at concentrations of 1, 10, 100, 1 000 and 10 000 mg/kg. Springtails were exposed to microplastics in standard soil for 28 days, after which the effect of microplastics was examined on organisms at the subcellular level of biological organization – activities of acetylcholinesterase (AChE), carboxylesterase (CES), glutathione S-transferase (GST) and catalase (CAT) were measured; and concentrations of glutathione (GSH), reactive oxygen species (ROS), carbohydrates and lipids. In both species, a significant change was observed in certain measured biomarkers, which can contribute to a better understanding of the impact of microplastics on soil organisms. |
