prikaz prve stranice dokumenta Poljedice interakcije vinove loze, arbuskularnih mikoriznih gljiva i virusa na primarni metabolizam
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undergraduate thesis
Poljedice interakcije vinove loze, arbuskularnih mikoriznih gljiva i virusa na primarni metabolizam
2024. urn:nbn:hr:181:530767
Nedeljković, Lovro
Josip Juraj Strossmayer University of Osijek
Department of biology

Cite this document

Nedeljković, L. (2024). Poljedice interakcije vinove loze, arbuskularnih mikoriznih gljiva i virusa na primarni metabolizam (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:530767

Nedeljković, Lovro. "Poljedice interakcije vinove loze, arbuskularnih mikoriznih gljiva i virusa na primarni metabolizam." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2024. https://urn.nsk.hr/urn:nbn:hr:181:530767

Nedeljković, Lovro. "Poljedice interakcije vinove loze, arbuskularnih mikoriznih gljiva i virusa na primarni metabolizam." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2024. https://urn.nsk.hr/urn:nbn:hr:181:530767

Nedeljković, L. (2024). 'Poljedice interakcije vinove loze, arbuskularnih mikoriznih gljiva i virusa na primarni metabolizam', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 16 November 2024, https://urn.nsk.hr/urn:nbn:hr:181:530767

Nedeljković L. Poljedice interakcije vinove loze, arbuskularnih mikoriznih gljiva i virusa na primarni metabolizam [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2024 [cited 2024 November 16] Available at: https://urn.nsk.hr/urn:nbn:hr:181:530767

L. Nedeljković, "Poljedice interakcije vinove loze, arbuskularnih mikoriznih gljiva i virusa na primarni metabolizam", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:181:530767

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Metadata
TitlePoljedice interakcije vinove loze, arbuskularnih mikoriznih gljiva i virusa na primarni metabolizam
Title (english)Consequences of grapevine, arbuscular mycorrhizal fungi and viruses interactions on primary metabolism
AuthorLovro Nedeljković
MentorRosemary Vuković (mentor)
Committee memberRosemary Vuković (predsjednik povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2024-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Uzgoj vinove loze, jedne od najznačajnijih poljoprivrednih kultura u Hrvatskoj, često je ugrožen pojavom različitih oboljenja koje uzrokuju virusi, utječući tako na urod i kvalitetu ploda. Jedna od ekološki prihvatljivih strategija za povećanje otpornosti biljaka na različite stresne čimbenike je primjena arbuskularnih mikoriznih (AM) gljiva u uzgoju. Cilj je ovog istraživanja utvrditi posljedice interakcije AM gljiva i virusa na primarni metabolizam vinove loze te odrediti potencijalnu ulogu metabolizma u obrambenom odgovoru vinove loze. Istraživanje je provedeno na vinovoj lozi sorte Merlot zaraženoj trima široko rasprostranjenim i/ili gospodarski važnim virusima (GRSPaV, GLRaV-3, GPGV) i inokuliranoj AM gljivama, a u konačnici je obuhvaćalo 15 skupina različitih kombinacija virusa, vinove loze i AM gljiva. Aktivnosti enzima uključenih u glikolizu, put pentoza-fosfata, biosintezu škroba i metabolizam saharoze određivane su spektofotometrijskim metodama poluvisoke propusnosti. Prilikom interakcije vinove loze, virusa i AM gljiva došlo je do promjene aktivnosti pojedinih enzima metabolizma ugljikohidrata u listu vinove loze. Zaraza virusima je u manjoj mjeri utjecala na metabolizam ugljikohidrata, a pojedine kombinacije virusa imale su različit učinak na enzime metabolizma. Inokulacija AM gljivama povećava sintezu saharoze te smanjuje sintezu škroba kao i aktivnost enzima uključenih u glikolizu u nezaraženim i zaraženim listovima vinove loze. Rezultati istraživanja doprinose razumijevanju uloge primarnog metabolizma u interakciji AM gljiva, vinove loze i virusa.
Abstract (english)
The cultivation of grapevine, one of the most important agricultural species in Croatia, is often endangered by various diseases caused by viruses, consequently affecting yield and fruit quality. Ecologically, one of the acceptable strategies for increasing a plant's resistance to different environmental stresses is using arbuscular mycorrhizal fungi (AMF) in cultivation. This study aims to determine the consequences of grapevine, AMF, and viruses interactions on grapevine primary metabolism and establish the potential role of AMF in grapevine defence response against viruses. The study was conducted on Merlot grapevine infected with three widespread and/or economically important viruses (GRSPaV, GLRaV-3, GPGV) and inoculated with AMF. It included 15 treatments with different combinations of grapevine, virus and AMF. Enzyme activities involved in glycolysis, pentose-phosphate pathway, starch biosynthesis and sucrose metabolism were analysed using spectrophotometric semi-high throughput methods. The interaction of grapevine, viruses and AMF caused changes in the activity of some enzymes involved in the carbohydrate metabolism of grapevine leaves. Virus infection had a minor impact on carbohydrate metabolism, and certain combinations of viruses affected metabolic enzymes differently. Inoculation with AMF increased sucrose synthesis and decreased starch synthesis, as well as the activities of enzymes involved in glycolysis in uninfected and infected grapevine leaves. Study results contribute to a better understanding of the role of primary metabolism in the interaction of AMF, virus and grapevine.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:530767
Study programmeTitle: Undergraduate University Study Programme in Biology (Bachelor of Science)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
Type of resourceText
File originBorn digital
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Created on2024-10-17 07:25:43