prikaz prve stranice dokumenta Odgovor klijanaca ozime pšenice na fiziološku sušu: mogućnost za rani probir osjetljivih i tolerantnih genotipova
Embargo period 2025-10-05
master's thesis
Odgovor klijanaca ozime pšenice na fiziološku sušu: mogućnost za rani probir osjetljivih i tolerantnih genotipova
2020. urn:nbn:hr:181:611928
Ament, Anita
Josip Juraj Strossmayer University of Osijek
Department of biology

Cite this document

Ament, A. (2020). Odgovor klijanaca ozime pšenice na fiziološku sušu: mogućnost za rani probir osjetljivih i tolerantnih genotipova (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:611928

Ament, Anita. "Odgovor klijanaca ozime pšenice na fiziološku sušu: mogućnost za rani probir osjetljivih i tolerantnih genotipova." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2020. https://urn.nsk.hr/urn:nbn:hr:181:611928

Ament, Anita. "Odgovor klijanaca ozime pšenice na fiziološku sušu: mogućnost za rani probir osjetljivih i tolerantnih genotipova." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2020. https://urn.nsk.hr/urn:nbn:hr:181:611928

Ament, A. (2020). 'Odgovor klijanaca ozime pšenice na fiziološku sušu: mogućnost za rani probir osjetljivih i tolerantnih genotipova', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 12 November 2024, https://urn.nsk.hr/urn:nbn:hr:181:611928

Ament A. Odgovor klijanaca ozime pšenice na fiziološku sušu: mogućnost za rani probir osjetljivih i tolerantnih genotipova [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2020 [cited 2024 November 12] Available at: https://urn.nsk.hr/urn:nbn:hr:181:611928

A. Ament, "Odgovor klijanaca ozime pšenice na fiziološku sušu: mogućnost za rani probir osjetljivih i tolerantnih genotipova", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:181:611928

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TitleOdgovor klijanaca ozime pšenice na fiziološku sušu: mogućnost za rani probir osjetljivih i tolerantnih genotipova
Title (english)REACTIONS OF WINTER WHEAT SEEDLINGS TO PHYSIOLOGICAL DROUGHT: THE POSSIBILITY OF EARLY SCREENING OF SENSITIVE AND TOLERANT GENOTYPES
AuthorAnita Ament
MentorVesna Peršić (mentor)
MentorJasenka Antunović Dunić (komentor)
Committee memberVera Cesar-Lepeduš (predsjednik povjerenstva)
Committee memberBranimir Hackenberger Kutuzović (član povjerenstva)
Committee memberJasenka Antunović Dunić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2020-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Osjetljivost biljaka na sušu genetski je predodređena u fenološkim, fiziološkim, biokemijskim i molekularnim svojstvima, a genetsko poboljšanje otpornosti zahtijeva identificiranje odgovarajućih mehanizama koji sudjeluju u inhibiranju ili ublažavanju štetnih učinaka stresa izazvanog nedostatkom vode. Tri dana stari klijanci 18 različitih sorti ozime pšenice izloženi su uvjetima vodnog deficita tijekom sedam dana (ψ = -0,3 MPa) uz pomoć polietilenglikola (PEG-6000). Istraživanje je obuhvatilo morfološke (visina izdanka i duljina korijena), fiziološke i biokemijske (relativni sadržaj vode, oštećenost biomembrana, razina lipidne peroksidacije, sadržaj slobodnog prolina, koncentracija fotosintetskih pigmenata) te fluorometrijske parametre (fluorescencija klorofila a). Multivarijantna analiza otkrila je da je potrebna potpuna interakcija složene kolekcije svojstava koje omogućuju prilagodbu na sušu kako bi se identificirali mehanizmi koji najbolje objašnjavaju varijabilnost među ispitivanim sortama. Fluorescencija klorofila a pokazala se kao najefikasnija metoda za rani probir osjetljivih i tolerantnih genotipova, ali ipak kombinacija parametara fluorescencije i određenih biokemijskih parametra ključna je za uspješnost oplemenjivačkog programa. Istraživanja utjecaja okolišnih stresora (poput suše) na rani rast klijanaca mogu upotpuniti znanja potrebna za rješavanje nekih temeljnih pitanja, kao što je stupanj plastičnosti, kako kod mladih nerazvijenih, tako i potpuno razvijenih biljaka.
Abstract (english)
Plant susceptibility to drought is genetically predetermined in phenological, physiological, biochemical, and molecular properties, and genetic improvement of drought resistance requires identification of appropriate mechanisms involved in inhibiting or mitigating the adverse effects of drought stress. Three-day-old seedlings of 18 different winter wheat cultivars were exposed to water deficit conditions for seven days (ψ = −0.3 MPa) using polyethylene glycol (PEG-6000). The study included measurements of morphological (shoot and root length), physiological and biochemical (relative water content, membrane leakage, lipid peroxidation level, free proline content, photosynthetic pigment concentration, and chlorophyll a fluorescence. Multivariate analysis revealed that the complete interaction of a complex collection of drought-adapting traits is needed to identify the parameters that best explain the variability among the studied cultivars. Chlorophyll a fluorescence appeared to be the most effective method for early screening of sensitive and tolerant genotypes. However, a combination of particular chlorophyll a fluorescence test parameters and specific biochemical parameters is vital for a successful breeding program. Research on the impact of environmental stressors (such as drought) on early seedling growth can complement the knowledge needed to address some fundamental issues, such as the degree of plasticity, in both young as well as fully developed plants.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:611928
Study programmeTitle: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology
Course: Master of Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-10-05
Terms of useLicence In copyright icon
Created on2020-10-05 09:55:43