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undergraduate thesis
Ekspresija gena za lignin u ječmu
2019. urn:nbn:hr:181:218305
Jarić, Bernard
Josip Juraj Strossmayer University of Osijek
Department of biology

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Jarić, B. (2019). Ekspresija gena za lignin u ječmu (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:218305

Jarić, Bernard. "Ekspresija gena za lignin u ječmu." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2019. https://urn.nsk.hr/urn:nbn:hr:181:218305

Jarić, Bernard. "Ekspresija gena za lignin u ječmu." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2019. https://urn.nsk.hr/urn:nbn:hr:181:218305

Jarić, B. (2019). 'Ekspresija gena za lignin u ječmu', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 28 December 2024, https://urn.nsk.hr/urn:nbn:hr:181:218305

Jarić B. Ekspresija gena za lignin u ječmu [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2019 [cited 2024 December 28] Available at: https://urn.nsk.hr/urn:nbn:hr:181:218305

B. Jarić, "Ekspresija gena za lignin u ječmu", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:181:218305

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Metadata
TitleEkspresija gena za lignin u ječmu
Title (english)Expression of genes involved in lignin synthesis in the barley stem
AuthorBernard Jarić
MentorLidija Begović (mentor)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2019-09-11, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Kao komponenta stanične stijenke lignin igra važnu ulogu u pružanju mehaničke potpore i transporta vode u biljkama. Sinteza lignina uključuje nekoliko putova. U ovom radu analizirali smo ekspresiju gena uključenih u sintezu lignina: fenilalanin amonijak-liazu (PAL), 4-kumarat: koenzim A ligazu (4CL) i reduktazu cinamoil-koenzima A (CCR) kao i celuloza sintetazu (CesA). Analize su provedene na tri kultivara ječma (Hordeum vulgare L.) u fazi nalijevanja zrna koristeći lančanu reakciju polimeraze u realnom vremenu (qPCR). Rezultati su pokazali da se ekspresija PAL, 4CL, CCR i CesA razlikuje u odnosu na referentni gen (ADP-ribozilacijski faktor 1-sličan protein, ADP). Najveća ekspresija zabilježena je u kultivaru Osvit, zatim slijedi kultivar Titan pa Predator koji je ujedno imao i najnižu ekspresiju svih gena.
Abstract (english)
As a part of the plant cell wall lignin plays an important roles providing mechanical support and water transport. Lignin synthesis includes several pathways. We analysed expression of genes involved in lignin synthesis: phenylalanine ammonia-lyase (PAL), 4-coumarate: CoA ligase (4CL) and cinnamoyl-CoA reductase (CCR) as well as cellulose synthase (CesA). Analyses were performed on three spring barley (Hordeum vulgare L.) cultivars at grain filling stage by using real-time polymerase chain reaction (qPCR). Results showed that expression of PAL, 4CL, CCR and CesA differed in comparison to the reference gene (ADP-ribosylation factor 1-like protein, ADP). The highest expression was observed in cultivar Osvit, followed by Titan and Predator which had the lowest expression of all genes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:218305
Study programmeTitle: Undergraduate University Study Programme in Biology (Bachelor of Science)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-12-10
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Created on2019-12-10 10:41:02