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master's thesis
ULOGA ADIPOKINETIČKOG HORMONA U OKSIDATIVNOM STRESU UZROKOVANOM OTROVOM BRAKONIDNE OSE HABROBRACON HEBETOR
2018. urn:nbn:hr:181:420088
Kljajić, Kristina
Josip Juraj Strossmayer University of Osijek
Department of biology

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Kljajić, K. (2018). ULOGA ADIPOKINETIČKOG HORMONA U OKSIDATIVNOM STRESU UZROKOVANOM OTROVOM BRAKONIDNE OSE HABROBRACON HEBETOR (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:420088

Kljajić, Kristina. "ULOGA ADIPOKINETIČKOG HORMONA U OKSIDATIVNOM STRESU UZROKOVANOM OTROVOM BRAKONIDNE OSE HABROBRACON HEBETOR." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2018. https://urn.nsk.hr/urn:nbn:hr:181:420088

Kljajić, Kristina. "ULOGA ADIPOKINETIČKOG HORMONA U OKSIDATIVNOM STRESU UZROKOVANOM OTROVOM BRAKONIDNE OSE HABROBRACON HEBETOR." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2018. https://urn.nsk.hr/urn:nbn:hr:181:420088

Kljajić, K. (2018). 'ULOGA ADIPOKINETIČKOG HORMONA U OKSIDATIVNOM STRESU UZROKOVANOM OTROVOM BRAKONIDNE OSE HABROBRACON HEBETOR', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 12 December 2024, https://urn.nsk.hr/urn:nbn:hr:181:420088

Kljajić K. ULOGA ADIPOKINETIČKOG HORMONA U OKSIDATIVNOM STRESU UZROKOVANOM OTROVOM BRAKONIDNE OSE HABROBRACON HEBETOR [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2018 [cited 2024 December 12] Available at: https://urn.nsk.hr/urn:nbn:hr:181:420088

K. Kljajić, "ULOGA ADIPOKINETIČKOG HORMONA U OKSIDATIVNOM STRESU UZROKOVANOM OTROVOM BRAKONIDNE OSE HABROBRACON HEBETOR", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:181:420088

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Metadata
TitleULOGA ADIPOKINETIČKOG HORMONA U OKSIDATIVNOM STRESU UZROKOVANOM OTROVOM BRAKONIDNE OSE HABROBRACON HEBETOR
Title (english)ROLE OF ADIPOKINETIC HORMONE IN OXIDATIVE STRESS ELICITED BY VENOM FROM THE BRACONID WASP HABROBRACON HEBETOR
AuthorKristina Kljajić
MentorNataša Turić (mentor)
Committee memberGoran Vignjević (predsjednik povjerenstva)
Committee memberNataša Turić (član povjerenstva)
Committee memberStjepan Krčmar (član povjerenstva)
Committee memberSandra Ečimović (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2018-10-31, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Glavni cilj rada je bio istražiti navodnu ulogu adipokinetičkog hormona u antioksidativnom odgovoru kod kukca Pyrrhocoris apterus tretiranog otrovom Habrobracon hebetor. Praćeni su biomarkeri oksidativnog stresa: sadržaj protein karbonila te aktivnosti glutation S-transferaze i katalaze. Kvantificiran je ukupan sadržaj adipokinetičkog hormona u središnjem živčanom sustavu. Ubrizgavanje otrova u plamenu stjenicu uzrokovalo je smanjenje sadržaja protein karbonila u hemolimfi ali je povećalo karbonilaciju probavila. Ko-tretmanom otrova i Pyrap-AKH sadržaj protein karbonila u hemolimfi se vratio na kontrolnu razinu ali se povećao iznad kontrolne razine u probavilu. Otrov je uzrokovao povećanje aktivnosti glutation S-transferaze u probavilu i umanjio aktivnost katalaze. Nakon kotretmana aktivnosti glutation S-transferaze i katalaze nisu se promjenile u odnosu na tretman otrovom što je neočekivano. Kvantifikacija Pyrap-AKH pokazala je povišene razine adipokinetičkog hormona u središnjem živčanom sustavu nakon ubrizgavanja otrova. Mišići tjelesne stjenke nisu reagirali na djelovanje otrova. Očito je da otrov uzrokuje oksidativni stres kod plamene stjenice ali rezultati nakon ko-tretmana otrova i adipokinetičkog hormona nisu potvrdili očekivanja
Abstract (english)
The main goal of the study is to explore this putative role of adipokinetic hormone in antioxidative stress response in the firebug Pyrrhocoris apterus body poisoned by Habrobracon hebetor venom. Oxidative stress biomarkers were monitored: protein carbonyl content and activity of glutathione Stransferase and catalase. Adipokinetic hormone level was quantified in central nervous system. Injection Habrobracon hebetor venom into Pyrrhocoris apterus body caused decrease in protein carbonyl content in hemolymph but increased carbonylation in midgut. When co-treated with venom and Pyrap-AKH, protein carbonyls returned to control level in hemolymph but increased above control level in midgut. Venom elicited activity of glutathione S-transferase in midgut but decreased catalase activity. After co-treatment glutathione S-transferase and catalase activity did not change compared to venom treatment which was not expected. Quantification of Pyrap-AKH showed elicited adipokinetic level in central nervous system after venom injection. Body wall muscles were not affected by venom. It is apparent that venom elicited oxidative stress in P. apterus but co-application of venom and adipokinetic hormone did not match the expectation
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:420088
Study programmeTitle: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology
Course: Master of Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-01-15 13:02:39