Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena  (Castanea sativa Mill.)

Topić, Katarina

prikaz prve stranice dokumenta Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.)

master's thesis

Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.)

2016. urn:nbn:hr:181:041970

Topić, Katarina

Josip Juraj Strossmayer University of Osijek
Department of biology

Cite this document

APA 6th Edition

Topić, K. (2016). Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.) (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:041970

MLA 8th Edition

Topić, Katarina. "Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.)." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2016. https://urn.nsk.hr/urn:nbn:hr:181:041970

Chicago 17th Edition

Harvard

Topić, K. (2016). 'Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.)', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:181:041970

Vancouver

Topić K. Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.) [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2016 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:181:041970

IEEE

K. Topić, "Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.)", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:181:041970

Cite this item: https://urn.nsk.hr/urn:nbn:hr:181:041970

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Metadata

Title	Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.)
Title (english)	CHARACTERIZATION OF CRYPHONECTRIA PARASITICA MURRILL BARR ISOLATES IN EUROPEAN CHESTNUT (CASTANEA SATIVA MILL.) CALLI
Author	Katarina Topić
Mentor	Ljiljana Krstin (mentor)
Committee member	Tanja Žuna Pfeiffer (predsjednik povjerenstva)
Committee member	Ljiljana Krstin (član povjerenstva)
Committee member	Dubravka Špoljarić Maronić (član povjerenstva)
Committee member	Filip Stević (član povjerenstva)
Granter	Josip Juraj Strossmayer University of Osijek Department of biology Osijek
Defense date and country	2016-09-09, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Fitopatogena gljiva Cryphonectria parasitica uzročnik je raka kore pitomog kestena Castanea sativa. Zaraza gljive hipovirusom, Cryphonectria hipovirus 1 (CHV1), smanjuje virulentnost i reproduktivni kapacitet gljive što doprinosi biološkoj kontroli raka kestenove kore. Hipovirulentna gljiva zaražena CHV1 virusom, unosom u aktivni rak uzrokovan virulentnim sojem gljive C. parasitica, omogućuje formiranje kalusa i zaustavlja širenje raka. Cilj ovog istraživanja bio je karakterizirati uzorke gljive C. parasitica na osnovi morfologije, tipa vegetativne kompatibilnosti (VC) i molekularnih analiza. Rezultati morfologije pokazali su prisutnost 55% žutih virulentnih sojeva i 45% bijelih sojeva gljive zaraženih hipovirusom. Najzastupljeniji vc tip gljive bio je EU2 (37%) zatim EU12 (27%), EU5 (18%) i EU1 (9%) dok je 9% bilo neodređenog VC tipa. U hipovirulentnim sojevima gljive dokazana je prisutnost hipovirusa CHV1 izolacijom dvolančane hipovirusne RNA i RT-PCR metodom, dok je analiza polimorfizma duljine restrikcijskih odsječaka (RFLP) potvrdila da CHV1 pripada talijanskom podtipu, CHV1-I.
Abstract (english)	Phytopathogenic fungus Cryphonectria parasitica is the causal agent of chestnut blight. Cryphonectria hypovirus 1 (CHV1) infects the fungal pathogen and reduces virulence and reproductive capacity of fungus which is important in the biological control of chestnut blight. If a hypovirulent fungal strain carrying CHV1 is introduced into an active canker caused by virulent C. parasitica strain, canker expansion ceases and callus is formed. The aim of this study was to characterize isolates of the fungus C. parasitica on the basis of morphology, type of vegetative compatibility and molecular analysis. Morphology results showed 55% yellow and 45% white hypovirulent strains. The most common VC type is the EU2 (37%) followed by the EU12 (27%), EU5 (18%) and EU1 (9%), while 9% were indeterminate VC type. Hypovirus CHV1 was found in all tested hypovirulent strains by dsRNA isolation and RT-PCR method, and the analysis of restriction fragment length polymorphism (RFLP) confirmed that CHV1 isolates belong to Italian subtype, CHV1-I.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:181:041970
Study programme	Title: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology Course: Master of Biology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
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Created on	2017-01-12 10:49:26