UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)

Pavičić, Martina

prikaz prve stranice dokumenta UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)

master's thesis

UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)

2013. urn:nbn:hr:181:455635

Pavičić, Martina

Josip Juraj Strossmayer University of Osijek
Department of biology

Cite this document

APA 6th Edition

Pavičić, M. (2013). UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.) (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:455635

MLA 8th Edition

Pavičić, Martina. "UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2013. https://urn.nsk.hr/urn:nbn:hr:181:455635

Chicago 17th Edition

Harvard

Pavičić, M. (2013). 'UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:181:455635

Vancouver

Pavičić M. UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.) [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2013 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:181:455635

IEEE

M. Pavičić, "UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:181:455635

Cite this item: https://urn.nsk.hr/urn:nbn:hr:181:455635

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Title	UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)
Title (english)	EFFECT OF ß-CRYPTOGEIN TRANSGENE ON PHENYLALANINE AMMONIA-LYASE ACTIVITY IN PAINTED NETTLE (Coleus blumei BENTH.) HAIRY ROOTS
Author	Martina Pavičić
Mentor	Elizabeta Has Schon (mentor)
Committee member	Ivna Štolfa Čamagajevac (predsjednik povjerenstva)
Committee member	Elizabeta Has Schon (član povjerenstva)
Committee member	Ljiljana Krstin (član povjerenstva)
Granter	Josip Juraj Strossmayer University of Osijek Department of biology Osijek
Defense date and country	2013-10-25, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Akumulacija ružmarinske kiseline (RA) u biljkama se često javlja kao dio obrambenog odgovora biljke na stres i napad patogena. Prvi korak biosinteze RA, neoksidativnu deaminaciju L-fenilalanina pri čemu nastaje trans-cimetna kiselina, katalizira enzim fenilalanin-amonij-lijaza (PAL). U staničnoj kulturi ukrasne koprive pojačanoj sintezi RA prethodi pojačana aktivnost PAL. Cilj ovog rada da bio je istražiti utjecaj endogeno sintetiziranog oomicetalnog elicitora ß-kriptogeina na aktivnost enzima PAL i biosintezu ružmarinske kiseline. U tu svrhu uzgojeno je transgeno korijenje ukrasne koprive (Coleus blumei Benth.), u kojem se sintetički gen crypt nalazi pod kontrolom inducibilnog alkoholnog promotora, te tretirano etanolom kako bi se inducirala sinteza -kriptogeina. Ekspresija ß-kriptogeina povećala je aktivnost enzima PAL, ali je uzrokovala značajno smanjenje sadržaja ružmarinske kiseline u linijama transgenog korijenja.
Abstract (english)	The accumulation of rosmarinic acid (RA) in plants often occurs as part of the plant defense response to stress and pathogen attack. The first step of RA biosynthesis, non-oxidative deamination of L-phenylalanine to trans-cinnamic acid, is catalyzed by the phenylalanine ammonia-lyase (PAL) enzyme. In cell culture of painted nettle enhanced synthesis of RA precedes to increased PAL activity. The aim of this study was to investigate the effect of endogenously synthesized oomycetal elicitor -cryptogein on the PAL enzyme activity and rosmarinic acid biosynthesis. For this purpose transgenic roots of painted nettle (Coleus blumei Benth.) were grown, in which a synthetic gene crypt was under the control of alcohol inducible promoter and treated with ethanol to induce synthesis of -cryptogein. Expression of -cryptogein increased the activity of PAL enzyme but caused a significant decrease of rosmarinic acid content in the genetic lines of transgenic roots.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:181:455635
Study programme	Title: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology Course: Master of Biology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
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Created on	2017-01-27 09:06:32