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master's thesis
Konstrukcija vektora za hetrolognu ekspresiju produkta gena At4g15590 iz biljke A. thaliana(L.) Heynh
2012. urn:nbn:hr:181:727613
Viandt, Krešimir
Josip Juraj Strossmayer University of Osijek
Department of biology

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Viandt, K. (2012). Konstrukcija vektora za hetrolognu ekspresiju produkta gena At4g15590 iz biljke A. thaliana(L.) Heynh (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:727613

Viandt, Krešimir. "Konstrukcija vektora za hetrolognu ekspresiju produkta gena At4g15590 iz biljke A. thaliana(L.) Heynh." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2012. https://urn.nsk.hr/urn:nbn:hr:181:727613

Viandt, Krešimir. "Konstrukcija vektora za hetrolognu ekspresiju produkta gena At4g15590 iz biljke A. thaliana(L.) Heynh." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2012. https://urn.nsk.hr/urn:nbn:hr:181:727613

Viandt, K. (2012). 'Konstrukcija vektora za hetrolognu ekspresiju produkta gena At4g15590 iz biljke A. thaliana(L.) Heynh', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 17 November 2024, https://urn.nsk.hr/urn:nbn:hr:181:727613

Viandt K. Konstrukcija vektora za hetrolognu ekspresiju produkta gena At4g15590 iz biljke A. thaliana(L.) Heynh [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2012 [cited 2024 November 17] Available at: https://urn.nsk.hr/urn:nbn:hr:181:727613

K. Viandt, "Konstrukcija vektora za hetrolognu ekspresiju produkta gena At4g15590 iz biljke A. thaliana(L.) Heynh", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:181:727613

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Metadata
TitleKonstrukcija vektora za hetrolognu ekspresiju produkta gena At4g15590 iz biljke A. thaliana(L.) Heynh
Title (english)Vector construction for heterologous expression At4g15590 gene product from plant A. thaliana(L.) Heynh
AuthorKrešimir Viandt
MentorHrvoje Fulgosi (mentor)
MBZ: 186771
Committee memberVera Cesar (predsjednik povjerenstva)
Committee memberHrvoje Fulgosi (član povjerenstva)
MBZ: 186771
Committee memberElizabeta Has Schon (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2012-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
U genomu biljke A. thaliana L. Heynh, identificiran je gen At4g15590 koji kodira suppressor of white apricot (SWAP) regulator prekrajanja (engl. splicing). Mikrosekvencioniranjem AM8 frakcijeizolirane iz cytb6f kompleksa iz špinata (S. oleracea) utvrđen je slijed PELDSSALV, koji pokazuje veliku homologiju sa slijedom PELDESALV u biljci A. thaliana (SWAP protein). Gen veličine 1677pb, koji je sastavljen je od 4 ili 5 egzona ovisno o višestrukim varijantama transkripata, na aminoterminalnom kraju gena karakterizira surp-domena, a na karboksiterminalnom kraju ubikvitin-domena.Metodom lančane reakcijom polimerazom (PCR) umnožen je dio gena SWAP, veličine 350 nukleotida, i ugrađen u plazmidni vektor pRSETa, gdje je fuziran na N-terminalnom kraju s peptidnim biljegom (6xHis tag). Ekspresija proteina ispitivana je u različitim bakterijskim stanicama pri različitim uvijetima uzgoja.
Abstract (english)
In plants A. thaliana L. Heynh genome a gene At4g15590 that codes suppressor of white apricot(SWAP) regulator for splicing has been discovered. Via microsequencing of AM8 fraction isolated from cytb6f spinach complex (S. oleracea) a sequence PELDSSALV has been established, a sequence that shows great hegemony with PELDESALV sequence in A. thaliana plant (SWAP protein). A 1677pb sized-gene, constructed from 4 or 5 exons, depending on multiple transcript variants, is characterized by a surp-domaine at the aminothermal gene region, and ubiquitin-domain at the carboxy-terminal region. Via polymerase chain reaction (PCR), SWAP part of the gene, 350 nucleotides in size, has been multiplied and built in a plasmidic pRSET vector where it has been fused on N-terminal end with peptide (6xHis) tag. Protein expresion has been researched in various bacterial cells under different bacteria breeding conditions.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:727613
Study programmeTitle: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology
Course: Master of Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-01-26 09:14:19