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master's thesis
Aktivnost MDR pumpi bakterija tla
2023. urn:nbn:hr:181:998991
Stanković, Josip
Josip Juraj Strossmayer University of Osijek
Department of biology

Cite this document

Stanković, J. (2023). Aktivnost MDR pumpi bakterija tla (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:998991

Stanković, Josip. "Aktivnost MDR pumpi bakterija tla." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2023. https://urn.nsk.hr/urn:nbn:hr:181:998991

Stanković, Josip. "Aktivnost MDR pumpi bakterija tla." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2023. https://urn.nsk.hr/urn:nbn:hr:181:998991

Stanković, J. (2023). 'Aktivnost MDR pumpi bakterija tla', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:181:998991

Stanković J. Aktivnost MDR pumpi bakterija tla [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2023 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:181:998991

J. Stanković, "Aktivnost MDR pumpi bakterija tla", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:181:998991

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Metadata
TitleAktivnost MDR pumpi bakterija tla
Title (english)MDR efflux pump activity in soil bacteria
AuthorJosip Stanković
MentorGoran Palijan (mentor)
Committee memberOlga Jovanović Glavaš (predsjednik povjerenstva)
Committee memberDavorka Hackenberger Kutuzović (član povjerenstva)
Committee memberGoran Palijan (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2023-10-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Antibiotska rezistentnost bakterija rastući je problem današnjice. Sve učestaliji mehanizam obrane bakterija je izbacivanje štetnih tvari pomoću membranskih proteina, odnosno efluks pomoću MDR („Multiple Drug Resistance”) pumpi. MDR efluks pumpe mogu izbacivati većinu klinički relevantnih antibiotika iz stanice u okoliš. Rezistentne bakterije koje eksprimiraju ove pumpe su općeprisutne u okolišu pa tako i tlima. Različiti mikroorganizmi koji se nalaze u tlu formiraju biofilmove koji im pomažu u obrani od antibiotika, a u samoj formaciji im pomažu MDR pumpe. Do danas su učinjena mnoga istraživanja na temu efluksa bakterija, no efluks mješovitih biofilmova iz tla je ostao gotovo ne istražen, a metode ograničene. U ovom istraživanju postavljene su nove metode uzgoja i formiranja biofilmova iz različitih uzoraka tla. Razvijene su nove metode mjerenja efluksa pomoću fluorescentnih boja FDA te rodamina 6G. Također, tijekom istraživanja efluksa korišteni su inhibitori efluksa; verapamil te karbonil cijanid mklorofenil hidrazon. U istraživanju su korišteni vlaženi i sušeni uzorci 8 tala različitog sastava i porijekla, a njihove sposobnosti efluksa međusobno uspoređene.
Abstract (english)
Antimicrobial resistance is a growing problem today. An increasingly frequent defense mechanism of bacteria is the expulsion of harmful substances using membrane proteins, i.e. efflux using MDR ("Multiple Drug Resistance") pumps. MDR efflux pumps can extrude majority of clinically relevant antibiotics from cell to environment. Resistant bacteria that express these pumps are ubiquitous in the environment, including soils. Different microorganisms found in the soil form biofilms that help them defend against antibiotics, and MDR pumps help them in the formation itself. To date, many studies have been carried out on the topic of bacterial efflux, but the efflux of mixed biofilms from the soil has remained almost unexplored, and the methods limited. In this research, new methods of cultivation and formation of biofilms from soil samples were established. New methods of measuring efflux using FDA and rhodamine 6G were developed. Also, efflux inhibitors were used during efflux research; verapamil and carbonyl cyanide m-chlorophenyl hydrazone. Wet and dried samples of 8 soils of different composition and origin were used in the research, and their efflux abilities were compared with each other.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:998991
Study programmeTitle: Master of Science Graduate University Study Programme in Nature and Environmental Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra Zaštite priode i okoliša (magistar/magistra Zaštite priode i okoliša)
Type of resourceText
File originBorn digital
Access conditionsClosed access
Terms of useLicence In copyright icon
Created on2023-11-02 09:43:12