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undergraduate thesis
Epigenetičke modifikacije genoma ptica
2023. urn:nbn:hr:181:206299
Beneš, Veronika
Josip Juraj Strossmayer University of Osijek
Department of biology

Cite this document

Beneš, V. (2023). Epigenetičke modifikacije genoma ptica (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:206299

Beneš, Veronika. "Epigenetičke modifikacije genoma ptica." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2023. https://urn.nsk.hr/urn:nbn:hr:181:206299

Beneš, Veronika. "Epigenetičke modifikacije genoma ptica." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2023. https://urn.nsk.hr/urn:nbn:hr:181:206299

Beneš, V. (2023). 'Epigenetičke modifikacije genoma ptica', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 24 April 2024, https://urn.nsk.hr/urn:nbn:hr:181:206299

Beneš V. Epigenetičke modifikacije genoma ptica [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2023 [cited 2024 April 24] Available at: https://urn.nsk.hr/urn:nbn:hr:181:206299

V. Beneš, "Epigenetičke modifikacije genoma ptica", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:181:206299

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Metadata
TitleEpigenetičke modifikacije genoma ptica
Title (english)Epigenetic modifications of the bird genome
AuthorVeronika Beneš
MentorLidija Begović (mentor)
MentorAlma Mikuška (komentor)
Committee memberLidija Begović (predsjednik povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2023-08-30, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Bijela roda (Ciconia ciconia L.) migratorna je vrsta iz porodice roda (Ciconiidae). Nastanjuje močvare, livade i šume. Hrani se većinom malim sisavcima, vodozemcima, gmazovima, kukcima i gujavicama. Antropogenim utjecajem bijela roda sve češće dolazi u kontakt s različitim izvorima onečišćenja koji se putem prehrane akumuliraju u hranidbeni lanac. Zagađenje okoliša i stres dovode do epigenetskih modifikacija koje posljedično uvjetuju promjenu u ekspresiji gena. U ovome radu određen je postotak metilacije regulatorne regije gena za metalotioneine (MT). Za određivanje postotka metilacije korišten je Metilacijski specifičan PCR u stvarnom vremenu (qMSP). Rezultati su pokazali značajnu statističku razliku za dva uzorka s područja s manjom okolišnom zagađenošću. Navedena dva uzorka ističu se sa manjim postotkom metilacije. Potrebna su daljnja istraživanja za potpuno razumijevanje utjecaja onečišćenja okoliša i teških metala na metilaciju i ekspresiju gena za metalotioneine kod bijele rode.
Abstract (english)
The white stork (Ciconia ciconia L.) is a migratory species from the Ciconiidae family. It inhabits swamps, meadows and forests. It feeds mostly on small mammals, amphibians, reptiles, insects and earthworms . Due to anthropogenic influence, the white stork more and more often comes into contact with different sources of pollution that accumulate in the food chain through food. Environmental pollution and stress lead to epigenetic modifications that consequently cause changes in gene expression. In this work, the methylation percentage of the regulatory region of the metallothionein (MT) gene was determined. Methylation-specific realtime PCR (qMSP) is used to determine the percentage of methylation. The results showed a significant statistical difference for two samples from areas with less environmental pollution. The mentioned two samples stand out with a lower percentage of methylation. Further research is needed to fully understand the impact of environmental pollution and heavy metals on methylation and metallothionein gene expression in white storks.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:206299
Study programmeTitle: Undergraduate University Study Programme in Biology (Bachelor of Science)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
Type of resourceText
File originBorn digital
Access conditionsClosed access
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Created on2023-09-12 06:29:13