prikaz prve stranice dokumenta Primjena metode bukalnog brisa u toksikologiji herpetofaune
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undergraduate thesis
Primjena metode bukalnog brisa u toksikologiji herpetofaune
2022. urn:nbn:hr:181:561850
Aleksandrić, Dino
Josip Juraj Strossmayer University of Osijek
Department of biology

Cite this document

Aleksandrić, D. (2022). Primjena metode bukalnog brisa u toksikologiji herpetofaune (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:561850

Aleksandrić, Dino. "Primjena metode bukalnog brisa u toksikologiji herpetofaune." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2022. https://urn.nsk.hr/urn:nbn:hr:181:561850

Aleksandrić, Dino. "Primjena metode bukalnog brisa u toksikologiji herpetofaune." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2022. https://urn.nsk.hr/urn:nbn:hr:181:561850

Aleksandrić, D. (2022). 'Primjena metode bukalnog brisa u toksikologiji herpetofaune', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 06 December 2024, https://urn.nsk.hr/urn:nbn:hr:181:561850

Aleksandrić D. Primjena metode bukalnog brisa u toksikologiji herpetofaune [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2022 [cited 2024 December 06] Available at: https://urn.nsk.hr/urn:nbn:hr:181:561850

D. Aleksandrić, "Primjena metode bukalnog brisa u toksikologiji herpetofaune", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:181:561850

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Metadata
TitlePrimjena metode bukalnog brisa u toksikologiji herpetofaune
Title (english)Application of buccal swabbing in toxicology of herpetofauna
AuthorDino Aleksandrić
MentorOlga Jovanović Glavaš (mentor)
MentorŽeljka Lončarić (komentor)
Committee memberOlga Jovanović Glavaš (predsjednik povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2022-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Ovaj završni rad bavi se testiranjem metode bukalnog brisa kao neinvazivne toksikološke metode. Zagađenje okoliša antropogenim utjecajem sve je veće stoga je i veća pažnja usmjerena na istraživanje utjecaja zagađenja na organizme i okoliš. Novijim istraživanjima nastoji se što manje je moguće zadirati u prirodu i na bilo koji način ju degradirati što daje na važnosti ovakvim metodama. One imaju sve širu primjenu u višim skupinama kralježnjaka (sisavci), ali na razini herpetofaune nisu još istražene. Tijekom istraživanja ulovljeno je 16 jedinki guštera (pet Lacerta agilis te 11 Podarcis muralis) kojima su uzeti brisevi. Uzorci su obrađeni i određivana je količina proteina. S obzirom na dobivene vrlo niske vrijednosti koncentracije proteina, enzimska aktivnost nije određivana. Metoda se pokazala nepouzdanom jer mala kolebanja u količini uzorka znatno utječu na rezultat. Uz to, kvalitetno uzimanje briseva gušterima je teško provedivo zbog nemogućnosti okretanja štapića kako bi se mogla uzet potrebna količina uzorka.
Abstract (english)
This bachelor thesis deals with testing the buccal swab method as a non-invasive toxicological method. Environmental pollution due to anthropogenic influence is increasing, therefore more attention is focused on researching the impact of pollution on organisms and the environment. Recent researches try to encroach on nature as little as possible and degrade it in any way, which gives importance to such methods. They are increasingly used in higher groups of vertebrates (mammals), but at the level of herpetofauna it has not yet been investigated. During the research, 16 lizards (five Lacerta agilis and 11 Podarcis muralis) were caught and their swabs were taken. The samples were processed and the amount of protein was determined. Due to the very low values of the protein concentration, the enzyme activity was not determined. The method proved to be unreliable because small fluctuations in the amount of sample significantly affect the result. In addition, high-quality swabbing of lizards is difficult due to the impossibility of turning the stick in order to take the required amount of sample.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:561850
Study programmeTitle: Undergraduate University Study Programme in Biology (Bachelor of Science)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
Type of resourceText
File originBorn digital
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Created on2022-11-02 11:35:26