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master's thesis
Utjecaj dva oblika selena (selenit i selenat) na imunološki sustav gujavice Eisenia andrei
2019. urn:nbn:hr:181:434284
Grgić, Magdalena
Josip Juraj Strossmayer University of Osijek
Department of biology

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Grgić, M. (2019). Utjecaj dva oblika selena (selenit i selenat) na imunološki sustav gujavice Eisenia andrei (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:434284

Grgić, Magdalena. "Utjecaj dva oblika selena (selenit i selenat) na imunološki sustav gujavice Eisenia andrei." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2019. https://urn.nsk.hr/urn:nbn:hr:181:434284

Grgić, Magdalena. "Utjecaj dva oblika selena (selenit i selenat) na imunološki sustav gujavice Eisenia andrei." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2019. https://urn.nsk.hr/urn:nbn:hr:181:434284

Grgić, M. (2019). 'Utjecaj dva oblika selena (selenit i selenat) na imunološki sustav gujavice Eisenia andrei', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 09 December 2024, https://urn.nsk.hr/urn:nbn:hr:181:434284

Grgić M. Utjecaj dva oblika selena (selenit i selenat) na imunološki sustav gujavice Eisenia andrei [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2019 [cited 2024 December 09] Available at: https://urn.nsk.hr/urn:nbn:hr:181:434284

M. Grgić, "Utjecaj dva oblika selena (selenit i selenat) na imunološki sustav gujavice Eisenia andrei", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:181:434284

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Metadata
TitleUtjecaj dva oblika selena (selenit i selenat) na imunološki sustav gujavice Eisenia andrei
Title (english)Effect of two different forms of selenium (selenite and selenate) on the immune system of the earthworm Eisenia andrei
AuthorMagdalena Grgić
MentorMirna Velki (mentor)
Committee memberSandra Ečimović (predsjednik povjerenstva)
Committee memberMirna Velki (član povjerenstva)
Committee memberSenka Blažetić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2019-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Selen je element u tragovima, potreban u malim količinama za normalno funkcioniranje animalnih organizama. Iako esencijalan, ukoliko je u organizmu prisutan u previsokim koncentracijama postaje toksičan te dovodi do razvoja bolesti. Unatoč tome, toksični utjecaj na organizme iz tla nije dovoljno istražen. Cilj ovog rada je stoga bio istražiti utjecaj dva oblika selena, selenita i selenata, na imunološki sustav gujavice Eisenia andrei, koristeći celomocite, efektorske stanice njezina imunološkog sustava. Kako bi se provjerila sposobnost obrane od patogena nakon izlaganja selenu, učinjen je test fagocitoze. Osim toga, izmjereni su sadržaji ROS-a i NO-a. Rezultati pokazuju da selen ne utječe negativno na sposobnost fagocitoze gujavica pri koncentraciji selenita, tj. selenata od 5 µg/cm2 te da ne utječe na promjenu u razini NO-a. Štoviše, pri toj koncentraciji selenat je utjecao na smanjenje sadržaja ROS-a u celomocitima.
Abstract (english)
Selenium is a trace element, required in small amounts for normal functioning of animal organisms. Even though essential, if present in concentration higher than necessary it becomes toxic and results in development of serious conditions. Despite this fact, researches concerning its toxicity effects on soil animals are scarce. The aim of this study was to investigate effects of two forms of selenium, selenite and selenate, on the immunological system of the earthworm Eisenia andrei, using coelomocytes, effector cells of its immunesystem. To assess earthworm’s ability of defence against pathogens after exposure to selenium, phagocytosis assay was done. Additionally, ROS and NO levels were measured. Results showed that selenium does not impair earthworm’s capability of phagocytosis or cause significant changes in NO levels. Moreover, at concentration of 5 µg/cm2 selenate causes decrease in ROS content in coelomocytes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:434284
Study programmeTitle: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology
Course: Master of Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-11-29
Terms of useLicence In copyright icon
Created on2020-02-03 08:10:50