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undergraduate thesis
Učinak selena na ekspresiju gena askorbat-glutationskog ciklusa kod pšenice (Triticum aestivum L.)
2019. urn:nbn:hr:181:805147
Sekulić, Ramona
Josip Juraj Strossmayer University of Osijek
Department of biology

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Sekulić, R. (2019). Učinak selena na ekspresiju gena askorbat-glutationskog ciklusa kod pšenice (Triticum aestivum L.) (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:805147

Sekulić, Ramona. "Učinak selena na ekspresiju gena askorbat-glutationskog ciklusa kod pšenice (Triticum aestivum L.)." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2019. https://urn.nsk.hr/urn:nbn:hr:181:805147

Sekulić, Ramona. "Učinak selena na ekspresiju gena askorbat-glutationskog ciklusa kod pšenice (Triticum aestivum L.)." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2019. https://urn.nsk.hr/urn:nbn:hr:181:805147

Sekulić, R. (2019). 'Učinak selena na ekspresiju gena askorbat-glutationskog ciklusa kod pšenice (Triticum aestivum L.)', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:181:805147

Sekulić R. Učinak selena na ekspresiju gena askorbat-glutationskog ciklusa kod pšenice (Triticum aestivum L.) [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2019 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:181:805147

R. Sekulić, "Učinak selena na ekspresiju gena askorbat-glutationskog ciklusa kod pšenice (Triticum aestivum L.)", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:181:805147

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Metadata
TitleUčinak selena na ekspresiju gena askorbat-glutationskog ciklusa kod pšenice (Triticum aestivum L.)
Title (english)The effect of selenium on the ascorbate-glutathione cycle genes expression in wheat (Triticum aestivum L.)
AuthorRamona Sekulić
MentorIvna Štolfa Čamagajevac (mentor)
MentorAna Vuković (pomoć pri izradi, neposredni voditelj)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2019-09-11, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Selen je mikronutrijent esencijalan za ljude i životinje, dok kod biljaka u malim koncentracijama djeluje u obrani protiv nepovoljnih ekoloških abiotičkih i biotičkih čimbenika. Pri većim koncentracijama selen može imati prooksidacijsko djelovanje i uzrokovati pojavu oksidacijskog stresa u stanicama. Važnu ulogu u smanjenju negativnih posljedica uzrokovanih oksidacijskim stresom ima askorbat-glutationski ciklusi te enzimi monodehidroaskorbat-reduktaza (MDHAR) i dehidroaskorbat-reduktaza (DHAR) koji čine njegov važan dio. U ovom istraživanju ispitivao se utjecaj tretmana različitih koncentracija i oblika selena (selenat i selenit) na razinu ekspresije gena mdhar i dhar u korijenu i izdanku pšenice (Triticum aestivum L., cv. Kraljica). Tretman selenom je značajno utjecao na ekspresiju gena mdhar i dhar pri čemu je razina ekspresije ovisila o kemijskom obliku i koncentraciji primijenjenog selna te o samom biljnom organu. Kako bi se detaljnije razjasnio mehanizam djelovanja selena na antioksidacijski odgovor u biljaka, potrebno je ispitati i ostale komponente antioksidacijskog sustava.
Abstract (english)
Selenium is a micronutrient essential for humans and animals, while in plants low concentrations of selenium help in protection against adverse ecological abiotic and biotic factors. At higher concentrations, selenium can have prooxidative activity and induce oxidative stress in cells. An important role in reducing the negative effects caused by oxidative stress has ascorbate-glutathione cycle and enzymes monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) that make its important part. In this study, the effect of various concentrations of selenium (selenate and selenium) on the expression of mdhar and dhar genes in the roots and shoots of wheat (Triticum aestivum L., cv. Kraljica) was studied. Treatment with selenium had a significant effect on the expression of mdhar and dhar genes, where the level of expression depended on the chemical form and concentration of the applied selenium and on the type of plant organ itself. In order to further clarify the mechanism of selenium on the antioxidant response in plants, other components of the antioxidant system need to be investigated.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:805147
Study programmeTitle: Undergraduate University Study Programme in Biology (Bachelor of Science)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-12-10
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Created on2019-12-10 11:11:07