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master's thesis
Utjecaj fungicida Pyrus 400 SC na ponašanje, aktivnost enzima i aktivnost efluks crpke u gujavici Eisenia andrei
2019. urn:nbn:hr:181:762536
Hmura, Marija
Josip Juraj Strossmayer University of Osijek
Department of biology

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Hmura, M. (2019). Utjecaj fungicida Pyrus 400 SC na ponašanje, aktivnost enzima i aktivnost efluks crpke u gujavici Eisenia andrei (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:762536

Hmura, Marija. "Utjecaj fungicida Pyrus 400 SC na ponašanje, aktivnost enzima i aktivnost efluks crpke u gujavici Eisenia andrei." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2019. https://urn.nsk.hr/urn:nbn:hr:181:762536

Hmura, Marija. "Utjecaj fungicida Pyrus 400 SC na ponašanje, aktivnost enzima i aktivnost efluks crpke u gujavici Eisenia andrei." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2019. https://urn.nsk.hr/urn:nbn:hr:181:762536

Hmura, M. (2019). 'Utjecaj fungicida Pyrus 400 SC na ponašanje, aktivnost enzima i aktivnost efluks crpke u gujavici Eisenia andrei', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:181:762536

Hmura M. Utjecaj fungicida Pyrus 400 SC na ponašanje, aktivnost enzima i aktivnost efluks crpke u gujavici Eisenia andrei [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2019 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:181:762536

M. Hmura, "Utjecaj fungicida Pyrus 400 SC na ponašanje, aktivnost enzima i aktivnost efluks crpke u gujavici Eisenia andrei", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:181:762536

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TitleUtjecaj fungicida Pyrus 400 SC na ponašanje, aktivnost enzima i aktivnost efluks crpke u gujavici Eisenia andrei
Title (english)Effect of fungicide Pyrus 400 SC on behavior, enzyme activity and efflux pump activity in earthworm Eisenia andrei
AuthorMarija Hmura
MentorMirna Velki (mentor)
Committee memberSandra Ečimović (predsjednik povjerenstva)
Committee memberMirna Velki (član povjerenstva)
Committee memberZorana Katanić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2019-09-19, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Fungicid Pyrus čija je glavna aktivna tvar pirimetanil, koncentracije 400 g/L, učinkovit je u suzbijanju sive plijesni, inhibira izlučivanje gljivičnih enzima, sprječava probavljanje biljnog tkiva i razvoj bolesti. Cilj ovog istraživanja bio je utvrditi učinak fungicida Pyrus na gujavice vrste Eisenia andrei. Određene su subletalne koncentracije fungicida koje ne uzrokuju morfološke promjene ni ugibanje gujavica, te je proveden test izbjegavanja, mjerene su aktivnosti enzima i aktivnost efluks crpke. Testom izbjegavanja utvrđeno je da gujavice izbjegavaju tlo tretirano fungicidom na svim ispitivanim koncentracijama. Mjerenjem promjena u aktivnosti različitih enzima, AChE, CAT, GR, CES i GST, uočeno je smanjenje aktivnosti AChE na svim koncentracijama. Aktivnosti ostalih enzima nisu pokazali značajnu promjenu u odnosu na kontrolu. Mjerenjem koncentracije Rodamina B utvrđena je inhibicija efluks crpke na nižim koncentracijama, odnosno dulje zadržavanje Rodamina B. Na većim koncentracijama zabilježena je indukcija aktivnosti efluks crpke, odnosno povećano izbacivanje Rodamina B. Rezultati pokazuju da fungicid Pyrus 400 SC utječe na mjerene parametre u gujavicama te da izlaganje ovom fungicidu može imati negativne učinke na gujavice.
Abstract (english)
Fungicide Pyrus contains pyrimethanil as active ingredient, concentration of 400 g/L, effective is in suppressing gray mold, inhibits the secretion of fungal enzymes blocking the ability of fungi to degrade and digest the plant tissues and thus stopping development of the disease. The overall aim of this study was to evaluate the effects of fungicide Pyrus on earthworms Eisenia andrei. Sublethal concentrations of fungicide that do not cause morfological changes or death in earhworms were determined, the avoidance test was performed and enzyme activity and efflux pump activity were measured. Avoidance test indicated that eartworms avoid treated soil at all tested concentrations. Measuring changes in the activity of different enzymes, AChE, CAT, GR, CES and GST, a decrease in AChE activity was observed at all concentrations. No statistically significant difference in the activity of other enzymes was recorded between control and fungicide treatments. Measuring the concentrations of Rodamin B revealed the inhibition of the efflux pump activity at lower concentrations, respectively, longer retention of Rodamin B. At higher concentrations the induction of the efflux pump activity was observed, respectively, the elimination of Rodamin B was enhanced. The results showed that the fungicide Pyrus 400 SC affects the measured parameters in earthworms and that exposure to this fungicide could have a harmful effects on earthworms.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:762536
Study programmeTitle: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology
Course: Master of Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-11-29
Terms of useLicence In copyright icon
Created on2019-11-29 11:40:10