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undergraduate thesis
Utjecaj vodnog stresa primjenom polietilen glikola na biljke u fazi klijanja i ranoj fazi rasta klijanaca
2018. urn:nbn:hr:181:722165
Ament, Anita
Josip Juraj Strossmayer University of Osijek
Department of biology

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Ament, A. (2018). Utjecaj vodnog stresa primjenom polietilen glikola na biljke u fazi klijanja i ranoj fazi rasta klijanaca (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:722165

Ament, Anita. "Utjecaj vodnog stresa primjenom polietilen glikola na biljke u fazi klijanja i ranoj fazi rasta klijanaca." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2018. https://urn.nsk.hr/urn:nbn:hr:181:722165

Ament, Anita. "Utjecaj vodnog stresa primjenom polietilen glikola na biljke u fazi klijanja i ranoj fazi rasta klijanaca." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2018. https://urn.nsk.hr/urn:nbn:hr:181:722165

Ament, A. (2018). 'Utjecaj vodnog stresa primjenom polietilen glikola na biljke u fazi klijanja i ranoj fazi rasta klijanaca', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:181:722165

Ament A. Utjecaj vodnog stresa primjenom polietilen glikola na biljke u fazi klijanja i ranoj fazi rasta klijanaca [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2018 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:181:722165

A. Ament, "Utjecaj vodnog stresa primjenom polietilen glikola na biljke u fazi klijanja i ranoj fazi rasta klijanaca", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:181:722165

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Metadata
TitleUtjecaj vodnog stresa primjenom polietilen glikola na biljke u fazi klijanja i ranoj fazi rasta klijanaca
Title (english)THE EFFECT OF POLYETHYLENE GLYCOL INDUCED WATER STRESS ON GERMINATION AND EARLY SEEDLING GROWTH
AuthorAnita Ament
MentorVesna Peršić (mentor)
MentorMartina Varga (komentor)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2018-07-17, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Vodni deficit je važan okolišni čimbenik koji utječe na klijavost, rast i razvoja biljaka te produktivnost poljoprivrednih kultura diljem svijeta. Cilj ovog istraživanja bio je razumjeti fiziološke i biokemijske mehanizme prilagodbe ozime pšenice sušnom stresu tijekom klijanja i ranog rasta klijanaca. Različite razine suše inducirane su korištenjem otopina polietilen glikola (PEG 6000) kojima su sjemenke tretirane tijekom šest dana. Za određivanje ukupne klijavosti i energije klijanja mjereni su postotak klijavosti, prosječno vrijeme klijanja, indeks stope klijavosti. Za utvrđivanje utjecaja suše na rast klijanaca mjerene su svježa masa klijanaca, duljina izdanka i korijena, sadržaj proteina i koncentracija slobodnog prolina kao i lipidna peroksidacija (udio malondialdehida) u korijenu i izdanku klijanaca, te koncentracija fotosintetskih pigmenata u izdanku. Rezultati ukazuju da deficit vode smanjuje parametre klijavosti i energiju klijanja u obje sorte. U uvjetima suše inducirane PEG-om dolazi do izraženijih fizioloških promjena u tkivu sorte El Nino u odnosu na sortu Felix. Povećanjem intenziteta suše inducirane PEG-om, El Nino akumulira dva puta više prolina u odnosu na sortu Felix. Dolazi do aktivacije mehanizama obrane od suše inducirane PEG-om, međutim ti mehanizmi nisu dovoljni da bi spriječili oštećenje membrana. Sorta Felix je pokazala veći potencijal tolerancije na sušu uzrokovanu PEG-om u fazi klijanja i ranog rasta klijanaca.
Abstract (english)
Soil water potential is an important environmental factor influencing germination, growth and development of plants as well as productivity of crop plants worldwide. The main purpose of this study was to understand the physiological and biochemical adaptation mechanisms of winter wheat trough germination stage and early seedling growth. Different levels of drought were induced by polyethylene glycol solution (PEG 6000) in which seeds were germinated and grown for six days. The final germination percentage, mean germination time and germination rate index were measured in order to determine total germination and germination energy. To determine the effect of drought on the seedlings growth fresh weight of seedlings, and shoot and root length were measured as well as lipid peroxidation (as malondialdehyde content) and osmotic substances (soluble proteins and free proline) in both shoots and roots of seedlings and photosynthetic pigments content in the shoots. The results indicated that reduced water potential decreased germination indicators and seedling vigour in both cultivars. When exposed to PEG-induced drought more intense physiological changes were determined in the El Nino plants in comparison to Felix. The defence mechanisms against negative effects of PEG-induced drought were activated. Nevertheless these mechanisms were insufficient to prevent membrane damage in the seedlings. The cultivar Felix showed better potential for PEG-induced drought tolerance in the germination stage and early growth stage.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:722165
Study programmeTitle: Undergraduate University Study Programme in Biology (Bachelor of Science)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-12-06 12:03:49