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master's thesis
Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.)
2016. urn:nbn:hr:181:041970
Topić, Katarina
Josip Juraj Strossmayer University of Osijek
Department of biology

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Topić, K. (2016). Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.) (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:041970

Topić, Katarina. "Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.)." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2016. https://urn.nsk.hr/urn:nbn:hr:181:041970

Topić, Katarina. "Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.)." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2016. https://urn.nsk.hr/urn:nbn:hr:181:041970

Topić, K. (2016). 'Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.)', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 14 April 2024, https://urn.nsk.hr/urn:nbn:hr:181:041970

Topić K. Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.) [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2016 [cited 2024 April 14] Available at: https://urn.nsk.hr/urn:nbn:hr:181:041970

K. Topić, "Karakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.)", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:181:041970

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Metadata
TitleKarakterizacija izolata gljive Cryphonectria parasitica Murrill Barr u kalusima raka kore pitomog kestena (Castanea sativa Mill.)
Title (english)CHARACTERIZATION OF CRYPHONECTRIA PARASITICA MURRILL BARR ISOLATES IN EUROPEAN CHESTNUT (CASTANEA SATIVA MILL.) CALLI
AuthorKatarina Topić
MentorLjiljana Krstin (mentor)
Committee memberTanja Žuna Pfeiffer (predsjednik povjerenstva)
Committee memberLjiljana Krstin (član povjerenstva)
Committee memberDubravka Špoljarić Maronić (član povjerenstva)
Committee memberFilip Stević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2016-09-09, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Fitopatogena gljiva Cryphonectria parasitica uzročnik je raka kore pitomog kestena Castanea sativa. Zaraza gljive hipovirusom, Cryphonectria hipovirus 1 (CHV1), smanjuje virulentnost i reproduktivni kapacitet gljive što doprinosi biološkoj kontroli raka kestenove kore. Hipovirulentna gljiva zaražena CHV1 virusom, unosom u aktivni rak uzrokovan virulentnim sojem gljive C. parasitica, omogućuje formiranje kalusa i zaustavlja širenje raka. Cilj ovog istraživanja bio je karakterizirati uzorke gljive C. parasitica na osnovi morfologije, tipa vegetativne kompatibilnosti (VC) i molekularnih analiza. Rezultati morfologije pokazali su prisutnost 55% žutih virulentnih sojeva i 45% bijelih sojeva gljive zaraženih hipovirusom. Najzastupljeniji vc tip gljive bio je EU2 (37%) zatim EU12 (27%), EU5 (18%) i EU1 (9%) dok je 9% bilo neodređenog VC tipa. U hipovirulentnim sojevima gljive dokazana je prisutnost hipovirusa CHV1 izolacijom dvolančane hipovirusne RNA i RT-PCR metodom, dok je analiza polimorfizma duljine restrikcijskih odsječaka (RFLP) potvrdila da CHV1 pripada talijanskom podtipu, CHV1-I.
Abstract (english)
Phytopathogenic fungus Cryphonectria parasitica is the causal agent of chestnut blight. Cryphonectria hypovirus 1 (CHV1) infects the fungal pathogen and reduces virulence and reproductive capacity of fungus which is important in the biological control of chestnut blight. If a hypovirulent fungal strain carrying CHV1 is introduced into an active canker caused by virulent C. parasitica strain, canker expansion ceases and callus is formed. The aim of this study was to characterize isolates of the fungus C. parasitica on the basis of morphology, type of vegetative compatibility and molecular analysis. Morphology results showed 55% yellow and 45% white hypovirulent strains. The most common VC type is the EU2 (37%) followed by the EU12 (27%), EU5 (18%) and EU1 (9%), while 9% were indeterminate VC type. Hypovirus CHV1 was found in all tested hypovirulent strains by dsRNA isolation and RT-PCR method, and the analysis of restriction fragment length polymorphism (RFLP) confirmed that CHV1 isolates belong to Italian subtype, CHV1-I.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:041970
Study programmeTitle: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology
Course: Master of Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-01-12 10:49:26