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undergraduate thesis
Biljne lipoksigenaze i njihova fiziološka uloga
2016. urn:nbn:hr:181:515594
Grgić, Magdalena
Josip Juraj Strossmayer University of Osijek
Department of biology

Cite this document

Grgić, M. (2016). Biljne lipoksigenaze i njihova fiziološka uloga (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:515594

Grgić, Magdalena. "Biljne lipoksigenaze i njihova fiziološka uloga." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2016. https://urn.nsk.hr/urn:nbn:hr:181:515594

Grgić, Magdalena. "Biljne lipoksigenaze i njihova fiziološka uloga." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2016. https://urn.nsk.hr/urn:nbn:hr:181:515594

Grgić, M. (2016). 'Biljne lipoksigenaze i njihova fiziološka uloga', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 04 December 2024, https://urn.nsk.hr/urn:nbn:hr:181:515594

Grgić M. Biljne lipoksigenaze i njihova fiziološka uloga [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2016 [cited 2024 December 04] Available at: https://urn.nsk.hr/urn:nbn:hr:181:515594

M. Grgić, "Biljne lipoksigenaze i njihova fiziološka uloga", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:181:515594

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Metadata
TitleBiljne lipoksigenaze i njihova fiziološka uloga
Title (english)Plant lipoxygenases and their physiological function
AuthorMagdalena Grgić
MentorElizabeta Has Schon (mentor)
Committee memberElizabeta Has Schon (predsjednik povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2016-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Selen kao mikronutrijent u biljkama može imati dvojno djelovanje. U malim količinama djeluje antioksidacijski, potičući aktivnost mnogih enzima koji sudjeluju u odgovoru na oksidacijski stres, dok u velikim količinama djeluje prooksidacijski i toksično. Cilj ovog rada bio je odrediti utjecaj različitih koncentracija selena u obliku selenita i selenata na ekspresiju lipoksigenaza (LOX) i razinu lipidne peroksidacije (LPO) u izdancima pšenice (Triticum aestivum L.). LOX su enzimi koji u biljci kataliziraju stvaranje hidroperoksida polinezasićenih masnih kiselina iz kojih daljnjim metaboličkim putevima nastaju oksilipini. Uloga oksilipina u stanici je značajna jer ih biljke sintetiziraju tijekom stresa, napada patogena ili biljojeda. Kada je biljka izložena stresu, količina LOX-a u biljci raste. U ovom istraživanju pšenica je tretirana različitim koncentracijama selena (0.01 mg × kg-1, 0.1 mg × kg-1 i 1 mg × kg-1) u obliku selenita i selenata, te su nakon 15 dana u izdancima mjereni ekspresija LOX enzima i reaktivnih supstanci tiobarbiturne kiseline (TBARS) kao pokazatelja razine LPO. Kod svih tretmana selenom, ekspresija LOX-a u izdancima bila je manja u odnosu na kontrolnu skupinu što ukazuje na negativni učinak selena na ekspresiju ovog enzima. Budući da količine TBARS-a u izdancima pšenice kod različitih tretmana nisu bile značajno veće u odnosu na kontrolu, možemo zaključiti da pri navedenim koncentracijama selen ni u jednom obliku ne izaziva pojačani oksidacijski stres u biljci.
Abstract (english)
As a micronutrient, selenium has a dual role in plants. At lower concentrations it acts as an antioxidant and stimulates oxidative stress response enzymes, whereas in higher concentrations it acts as a pro-oxidant and it is toxic. The aim of this study was to determine the effects of different forms and concentractions of selenium on the expression of plant lypoxigenases (LOX) and lipid peroxidation levels (LPO) in the wheat shoots (Triticum aestivum L.). LOX enzymes catalyze the formation of unsaturated fatty acid hydroperoxide from polyunsaturated fatty acids, from which in further metabolic pathways oxylipins are formed. Oxylipins have significant role in plants because they are synthesised in response to stress, pathogen or herbivore attack. In plants subjected to stress LOX levels are increased. In this research, wheat was treated with different concentrations (0.01 mg × kg-1, 0.1 mg × kg-1 i 1 mg × kg-1) of selenite or selenate and after 15 days, expression of LOX and LPO levels, expressed as Thiobarbituric Acid Reactive Substances (TBARS), in shoots were measured. All plants treated with both selenium forms, selenite and selenate, showed decreased levels of LOX with respect to control, indicating negative correlation between selenium treatment and LOX expression. Since the level of TBARS in shoots were not statistically different compared to control, we can conclude that selenium in neither of its forms and applied concentrations induced stress response in plants.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:515594
Study programmeTitle: Undergraduate University Study Programme in Biology (Bachelor of Science)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2017-01-31 08:47:53